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An Optimization of a technique for the isolation of  5- HT Positive en  erochromaffin cell from the GL tract and examination of how vesicular 5-ht release is affected by age.

Aim of the inv estigation:

To optimize EC cells isolation from mouse colon

To characterize the effects of age on 5-HT release

properties from murine enterochromaffin cells using electro chemical-sensing

Main objectives

1) To investigate and understand the

function of EC cells in GI tract

2% The way in which motility occurs with age and the role of EC cell in this.

3 The role of serotonin on

these cells

Specific objective

Ex animating different age group mouse of 3, 18 and 24 Months by;

1) Ev oke inherent mucosal reflexes

gestehd by brush stimulation or circumferential stretch. (To understand the changes occur in proximal and distal colon when stimulated

rus
2) Recording from electrical field stimulation of segments of intact distal colon with and without mucosa.